Optimization Carotenoids Isolation Of The Waste Crude Palm Oil Using -Amylase, -Amylase, And Cellulase

Waste oil industry has a great potential use, as 77% oil yield are an untapped waste that still has a high economic value as a base for the oleochemical industry, one of which is β-carotene. The study was conducted to optimize the extraction of carotenoids from Crude Palm Oil (CPO) and Palm Oil Mill Effluent waste (POME) conducted by column chromatography and extraction method with a single enzyme : -amylase, -amylase, or cellulase and its mixed. In addition, the saponification reaction performed on CPO to separate unsaponifiable fraction by the addition of enzyme pretreatment. The results showed that the extraction of carotenoids with the help of enzymes better than separation prossses using the column chromatography. Amylase, -amylase, and cellulase may help release carotenoids from the matrix of fibers and other carbohydrates in the CPO and POME waste. Optimum conditions -amylase obtained at 70C and pH 6.0, amylase at 60 C and pH 4.0, cellulase at 40 C and pH 7.0. While the use of a mixture of cellulose : -amylase : -amylase with a ratio of 1 : 1 : 2 at pH 5.5 and temperature 60 C that yielded optimal -carotene. Extractions with enzymatic methods of waste palm oil were able to isolate -carotene of 0.19 % equivalent to 1160 ppm. Keyword: -caroten, CPO and POME, column chromatography,and enzymatic extraction.